RE9 Blood Specimen Analyzer Puzzle — 4×4 Vial Solution

Author note: Written by Jachin, a 23-year Resident Evil series fan based in Seoul, South Korea. This guide is based on 6 full RE9 playthroughs on PS5 and Steam (Casual → Insanity → NG+ Insanity completions). All strategies, timings, and locations have been personally tested across the v1.02 patch. AI tools were used only for grammar review and note organization; no AI-generated content appears in strategy analysis.

Verified on: v1.02 + DLC patch · Last reviewed: May 30, 2026 · About the author · Editorial policies

PUBLISHED MAY 2026 · 6 MIN READ · DIFFICULTY: ★★★ MEDIUM-HARD · NO STORY SPOILERS
✓ SPOILER LEVEL: LOW

RE9's Blood Specimen Analyzer is a 4×4 grid puzzle in the ARK Facility Sterilization Chamber. You need to match 4 blood-type vials to the correct slots using the blood-type compatibility chart found on the wall. Get it wrong twice and the chamber locks. Full solution + chart explained below.

LocationARK Facility — Sterilization Chamber (B2)
Difficulty★★★ Medium-Hard (chart reading required)
Puzzle type4×4 grid placement
Failure penaltyChamber locks for 60 sec after 2 wrong tries
RewardLab Access Card B + 1 First Aid Spray
Verified onv1.02
Table of contents

How the analyzer works

The Blood Specimen Analyzer sits on a steel pedestal in the center of the Sterilization Chamber. It has 4 slots arranged left-to-right (positions 1, 2, 3, 4) and you pick up 4 blood vials from the fridge along the north wall. Each vial is labeled with a blood type (A+, A−, B+, B−, O+, O−, AB+, AB−).

On the south wall there's a faded chart showing which blood type can transfuse into which (the universal donor/recipient rules). Your job is to place 4 vials so that each vial in position N can transfuse into the vial in position N+1 — i.e. a "donation chain" from left to right.

⚠ COMMON MISTAKE: Players try to place all 4 of the same blood type. The chart is about donation compatibility, not matching types. Read the section below before you place anything.

Blood-type chart (decoded)

Here's the chart on the wall, simplified. An arrow A → B means "type A can donate to type B".

TypeCan donate toCan receive from
O− (universal donor)EveryoneO− only
O+O+, A+, B+, AB+O+, O−
A−A−, A+, AB−, AB+A−, O−
A+A+, AB+A+, A−, O+, O−
B−B−, B+, AB−, AB+B−, O−
B+B+, AB+B+, B−, O+, O−
AB−AB−, AB+AB−, A−, B−, O−
AB+ (universal recipient)AB+ onlyEveryone

The solution (4 vials, left to right)

You need a donation chain. The intended (and only) chain that uses the 4 vials Grace finds in the fridge is:

✓ CORRECT ORDER (left → right)
Slot 1: O−Slot 2: A−Slot 3: A+Slot 4: AB+

Why this works:

Step-by-step walkthrough

  1. Enter Sterilization Chamber via the Lab Access Card A door (Lower Lab → west corridor).
  2. Pick up all 4 vials from the fridge on the north wall. The fridge contains: O−, A−, A+, AB+. You don't need any other vials.
  3. Read the blood-type chart on the south wall (or just use the solution above).
  4. Walk to the analyzer pedestal in the center.
  5. Place vials in this order: Slot 1 = O−, Slot 2 = A−, Slot 3 = A+, Slot 4 = AB+.
  6. Press the green Analyze button. Chamber hums for 8 sec, then lights turn green.
  7. Lab Access Card B drops from a slot on the right side of the pedestal. Pick it up.
  8. First Aid Spray spawns on the analyzer's top tray. Grab it.
  9. Card B opens the next door — leads to the Quartz Puzzle Hub.

Tips & what NOT to do

  1. Don't waste vials on wrong slots — placing a vial in the wrong slot doesn't consume it, but 2 wrong configurations triggers a 60-sec lockout. Use the chart.
  2. O− goes in slot 1 always — universal donor is the only vial that can sit at the start of any chain.
  3. AB+ goes in slot 4 always — universal recipient is the only vial that can sit at the end.
  4. Slots 2 and 3 must bridge — A−/A+ pair is the only valid bridge with the vials Grace finds.
  5. Insanity mode bonus: solving on the first try awards a Hemorrhetic Injector in the analyzer's bottom drawer (skip if you got 1+ wrong tries).
  6. NG+ wisdom: the fridge contents don't change between difficulties — the solution is always O− → A− → A+ → AB+.
Related puzzles
Eye Spy Charm explained Organless corpse puzzle Puzzle box solutions All quartz puzzle solutions ARK Facility walkthrough All guides
All 25 Mr. Raccoon Locations How to Beat Victor Gideon How to Beat Super Tyrant All 5 Safe Codes All guides home

Was this guide helpful? Let us know — we update tier rankings monthly based on community feedback and balance patches.

If this guide helped, consider supporting more guides like this on Ko-fi. Every little bit means the world.

☕ Support on Ko-fi